Anti-ATF6 antibody [1-7] (ab122897)
![Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.com/ps/products/122/ab122897/Images/ab122897-275675-anti-atf6-antibody-1-7-western-blot.jpg "Anti-ATF6 antibody [1-7] (ab122897)")
Key features and details
- Mouse monoclonal [1-7] to ATF6
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ATF6 antibody [1-7]
See all ATF6 primary antibodies -
Description
Mouse monoclonal [1-7] to ATF6 -
Host species
Mouse -
Specificity
specific to human ATF6 alfa no cross reactivity with mouse ATF6 alfa. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human ATF6 (N terminal). Epitope is not determined
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Positive control
- 293T, HeLa S3 Tet-off, 293 and HeLa cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purification notes
ab122897 was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes, then filter sterilized. -
Clonality
Monoclonal -
Clone number
1-7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-ATF6 antibody [1-7] (ab122897)
Lane 1: Wild type HAP1 whole cell lysate (50 µg)
Lane 2: ATF6 knockout HAP1 whole cell lysate (50 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab122897 observed at 95 kDa. Red - loading control, ab181602, observed at 37 kDa.ab122897 was shown to specifically recact with ATF6 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ATF6 knockout samples were used. Wild-type and ATF6 knockout samples were subjected to SDS-PAGE. Ab122897 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.com/ps/products/122/ab122897/Images/ab122897-253198-anti-atf6-antibody-1-7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody [1-7] (ab122897)Immunofluorescent analysis of HeLa cells using ab122897 at a dilution of 1/100. The cells were fixed with paraformaldehyde. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG at a dilution of 1/1000. The antibody staining is shown in the top panel, and the merged image with DAPI counter-staining is shown in the lower panel.
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![Western blot - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.com/ps/products/122/ab122897/Images/ab122897-136203-anti-atf6-antibody-1-7-western-blot.jpg)
Western blot - Anti-ATF6 antibody [1-7] (ab122897)All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution
Lane 1 : 293T cell lysate: control
Lane 2 : 293T cell lysate: treated with 2 µg/ml tunicamycin for 7 hours
Lane 3 : 293T cell lysate: treated with 1mM DTT for 1 hour.
Lane 4 : HeLa S3 Tet-off cell lysate: control
Lane 5 : HeLa S3 Tet-off cell lysate: treated with 1mM DTT for 1 hour.
Predicted band size: 75 kDa
Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells. -
![Western blot - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.com/ps/products/122/ab122897/Images/ab122897-136202-anti-atf6-antibody-1-7-western-blot.jpg)
Western blot - Anti-ATF6 antibody [1-7] (ab122897)All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilution
Lane 1 : HeLa cell extract: control
Lane 2 : HeLa cell extract: treated with 1mM DTT for 1 hour
Lane 3 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 3 hour
Lane 4 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 7 hour
Lane 5 : 293 cell extract: control
Lane 6 : 293 cell extract: treated with 1mM DTT for 1 hour
Lane 7 : 293 cell extract: treated with 2 µg/ml tunicamycin for 3 hour
Lane 8 : 293 cell extract: treated with 2 µg/ml tunicamycin for 7 hour
Predicted band size: 75 kDa
Conversion of precursors pATF6(P) to pATF6(N) in DTT- or tunicamycin-treated cells. ATF6 is constitutively expressed as pATF6a(P) (~90-kDa protein), and converted to pATF6a(N) (>50-kDa protein) in ER-stressed cells. -
![Immunoprecipitation - Anti-ATF6 antibody [1-7] (ab122897)](https://www.abcam.com/ps/products/122/ab122897/Images/ab122897-136201-anti-atf6-antibody-1-7-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ATF6 antibody [1-7] (ab122897)ATF6 was detected by Western blot (Input; lanes 1, 2, 7, and 8) using ab122897 (No. 1-7). After immunoprecipitation (IP) with non-related IgG (IP; lanes 3, 4, 9, and 10) or ab122897 (No. 1-7) (IP; lanes 5, 6, 11, and 12), samples were subjected to SDS-PAGE and analyzed by Western blot using ab122897 (No. 1-7) and anti-mouse IgG antibody (light chain specific).
