Anti-CCR3 antibody [Y31] (ab32512)
![Anti-CCR3 antibody [Y31] (ab32512)](https://www.abcam.com/ps/products/32/ab32512/Images/ab32512-45066-anti-ccr3-antibody-y31-immunohistochemistry.jpg "Anti-CCR3 antibody [Y31] (ab32512)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y31] to CCR3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CCR3 antibody [Y31]
See all CCR3 primary antibodies -
Description
Rabbit monoclonal [Y31] to CCR3 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human CCR3 (C terminal). The exact sequence is proprietary.
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Positive control
- K562 and Daudi cell lysates and human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y31 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] (ab32512)
Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-CCR3 antibody [Y31] (ab32512)](https://www.abcam.com/ps/products/32/ab32512/Images/ab32512-45065-anti-ccr3-antibody-y31-western-blot.jpg)
Western blot - Anti-CCR3 antibody [Y31] (ab32512)All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : Daudi cell lysate
Predicted band size: 43 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Anti-CCR3 antibody [Y31] (ab32512)](https://www.abcam.com/ps/products/32/ab32512/Images/ab32512-3-benefits-of-recombinant-antibodies.png)
Anti-CCR3 antibody [Y31] (ab32512)
