Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23502-85] to CCR4 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free
    See all CCR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23502-85] to CCR4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: HDLM-2, and PBMC cells; HEK-293T cells transfected with GFP tagged CCR4 overexpression vector.

  • General notes

    ab275400 is the carrier-free version of ab254376. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275400 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
    Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)

    This data was developed using ab254376, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR4 with ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable lymphocytes

  • Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
    Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)

    This data was developed using ab254376, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged CCR4 overexpression vector) cells labeling CCR4 with ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells. 

  • Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
    Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)

    This data was developed using ab254376, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HDLM-2 (human Hodgkin lymphoma, Right) cells labeling CCR4 with ab254376 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293 (PMID: 22973452).

    Gated on viable cells.

  • Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
    Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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