Anti-CCR3 antibody [5E8] (ab16231)
![Anti-CCR3 antibody [5E8] (ab16231)](https://www.abcam.com/ps/products/16/ab16231/Images/ab16231-24896-anti-ccr3-antibody-5e8-immunofluorescence.jpg "Anti-CCR3 antibody [5E8] (ab16231)")
Key features and details
- Mouse monoclonal [5E8] to CCR3
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CCR3 antibody [5E8]
See all CCR3 primary antibodies -
Description
Mouse monoclonal [5E8] to CCR3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human) - human CCR3 transfected into mouse B cell lymphoma L1.2 cells.
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Positive control
- Blood eosinophils and basophils.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5E8 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [5E8] (ab16231)ICC/IF image of ab16231 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
