Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CCR6 antibody [EPR22259] (ab227036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22259] to CCR6
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CCR6 antibody [EPR22259]
See all CCR6 primary antibodies
Description

Rabbit monoclonal [EPR22259] to CCR6
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HL-60, Hut-78 and HUVEC whole cell lysates; Human tonsil and thymus tissue lysate. IHC-P: Human Hodgkin's lymphoma and tonsil tissue. IP: HL-60 whole cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22259
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] (ab227036)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human tonsil (PMID: 12514792) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] (ab227036)

Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human Hodgkin’s lymphoma (PMID: 12514792) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-CCR6 antibody [EPR22259] (ab227036)

All lanes : Anti-CCR6 antibody [EPR22259] (ab227036) at 1/1000 dilution

Lane 1 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lane 2 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 3 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 4 : Human tonsil tissue lysate
Lane 5 : Human thymus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Predicted band size: 42 kDa

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-CCR6 antibody [EPR22259] (ab227036)

CCR6 was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with ab227036 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab227036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1: HL-60 whole cell lysate 10 µg (input).
Lane 2: ab227036 IP in HL-60 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227036 in HL-60 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Anti-CCR6 antibody [EPR22259] (ab227036)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com