Anti-CCR6 antibody [MM0066-3L1] (ab93086)
![Anti-CCR6 antibody [MM0066-3L1] (ab93086)](https://www.abcam.com/ps/products/93/ab93086/Images/ab93086-108221-anti-ccr6-antibody-mm0066-3l1-flow-cytometry.jpg "Anti-CCR6 antibody [MM0066-3L1] (ab93086)")
Key features and details
- Mouse monoclonal [MM0066-3L1] to CCR6
- Suitable for: IHC-P, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-CCR6 antibody [MM0066-3L1]
See all CCR6 primary antibodies -
Description
Mouse monoclonal [MM0066-3L1] to CCR6 -
Host species
Mouse -
Specificity
This antibody detects CCR6, but not other CCRs. -
Tested applications
Suitable for: IHC-P, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CCR6. 293 cells transfected with Human CCR6.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered. -
Clonality
Monoclonal -
Clone number
MM0066-3L1 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - Anti-CCR6 antibody [MM0066-3L1] (ab93086)Overlay histogram showing Jurkat cells stained with ab93086 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93086, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (ab93086)](https://www.abcam.com/ps/products/93/ab93086/Images/ab93086-242335-anti-ccr6-antibody-mm0066-3l1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (ab93086)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCR6 with ab93086. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
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![Immunohistochemistry paraffin embedded sections - Anti-CCR6 antibody [MM0066-3L1] (ab93086)](https://www.abcam.com/ps/products/93/ab93086/Images/ab93086-108222-anti-ccr6-antibody-mm0066-3l1-immunohistochemistry.jpg)
Immunohistochemistry paraffin embedded sections - Anti-CCR6 antibody [MM0066-3L1] (ab93086)IHC image of ab93086 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93086, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
