Anti-CCR7 antibody [E75] (ab32075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E75] to CCR7
- Suitable for: ELISA, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-CCR7 antibody [E75]
See all CCR7 primary antibodies -
Description
Rabbit monoclonal [E75] to CCR7 -
Host species
Rabbit -
Specificity
ab32075 recognises CCR7/CKR7. It does not cross react with other CKR family members -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB MouseRatHuman -
Immunogen
A synthetic peptide corresponding to residues in loop-3 extracellular domain of human C-C chemokine receptor type 7
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Positive control
- Daudi cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
E75 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : Human lung lysate
Lane 3 : Human lymph node lysate
Lane 4 : Human spleen lysate
Lane 5 : Human plasma lysate
Lane 6 : Human brain lysate (low/negative expression)
Lane 7 : Human liver lysate (low/negative expression)
Lane 8 : Mouse lung lysate
Lane 9 : Mouse lymph node lysate
Lane 10 : Mouse spleen lysate
Lane 11 : Mouse plasma lysate
Lane 12 : Rat lung lysate
Lane 13 : Rat lymph node
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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