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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)

Anti-CCR7 antibody [E75] (ab32075)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CCR7 antibody [E75] (ab32075)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E75] to CCR7
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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Overview

  • Product name

    Anti-CCR7 antibody [E75]
    See all CCR7 primary antibodies
  • Description

    Rabbit monoclonal [E75] to CCR7
  • Host species

    Rabbit
  • Specificity

    ab32075 recognises CCR7/CKR7. It does not cross react with other CKR family members
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    A synthetic peptide corresponding to residues in loop-3 extracellular domain of human C-C chemokine receptor type 7

  • Positive control

    • Daudi cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    E75
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Images

  • Western blot - Anti-CCR7 antibody [E75] (ab32075)
    Western blot - Anti-CCR7 antibody [E75] (ab32075)
    All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : Human lung lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Human spleen lysate
    Lane 5 : Human plasma lysate
    Lane 6 : Human brain lysate (low/negative expression)
    Lane 7 : Human liver lysate (low/negative expression)
    Lane 8 : Mouse lung lysate
    Lane 9 : Mouse lymph node lysate
    Lane 10 : Mouse spleen lysate
    Lane 11 : Mouse plasma lysate
    Lane 12 : Rat lung lysate
    Lane 13 : Rat lymph node

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CCR7 antibody [E75] (ab32075)
    Western blot - Anti-CCR7 antibody [E75] (ab32075)
    Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • ELISA - Anti-CCR7 antibody [E75] (ab32075)
    ELISA - Anti-CCR7 antibody [E75] (ab32075)
    ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-CCR7 antibody [E75] (ab32075)
    Anti-CCR7 antibody [E75] (ab32075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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