HRP Anti-CCR7 antibody [Y59] (ab196475)
![HRP Anti-CCR7 antibody [Y59] (ab196475)](https://www.abcam.com/ps/products/196/ab196475/Images/ab196475-388316-196475jfle0632westernblotc6raw2647.jpg "HRP Anti-CCR7 antibody [Y59] (ab196475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y59] to CCR7
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Overview
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Product name
HRP Anti-CCR7 antibody [Y59]
See all CCR7 primary antibodies -
Description
HRP Rabbit monoclonal [Y59] to CCR7 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6 and Raw264.7 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y59 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-CCR7 antibody [Y59] (ab196475)All lanes : HRP Anti-CCR7 antibody [Y59] (ab196475) at 1/5000 dilution (Recombinant Anti-CCR7 antibody [Y59] (HRP))
Lane 1 : C6 (rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Exposure time: 10 secondsab196475 was shown to react with CCR7 in western blot. Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab196475 overnight at 4°C at a 1 in 5000 dilution. Blots were incubated with secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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![HRP Anti-CCR7 antibody [Y59] (ab196475)](https://www.abcam.com/ps/products/196/ab196475/Images/ab196475-4-benefits-of-recombinant-antibodies.png)
HRP Anti-CCR7 antibody [Y59] (ab196475)
