Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR7 antibody [Y59] (ab32527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y59] to CCR7
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human, African green monkey

Overview

  • Product name

    Anti-CCR7 antibody [Y59]
    See all CCR7 primary antibodies

  • Description

    Rabbit monoclonal [Y59] to CCR7

  • Host species

    Rabbit

  • Specificity

    The antibody does not cross-react with other G-protein coupled receptor 1 family members.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    African green monkey
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CCR7 (N terminal). The exact sequence is proprietary.
    Database link: P32248

  • Positive control

    • WB: COS-1, C6, RAW 264.7 and PC-12 cell lysates. ICC/IF: Jurkat cells. IP: MCF7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CCR7 antibody [Y59] (ab32527)
    Western blot - Anti-CCR7 antibody [Y59] (ab32527)
    All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : COS-1 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 2 : C6 (rat glial tumor cell line) cell lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 43 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)
    Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] (ab32527)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human  T cell leukemia cell line from peripheral blood) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)
    Immunoprecipitation - Anti-CCR7 antibody [Y59] (ab32527)

    ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF7 (human breast adenocarcinoma cell line) whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CCR7 antibody [Y59] (ab32527)
    Anti-CCR7 antibody [Y59] (ab32527)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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