Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)
![Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)](https://www.abcam.com/ps/products/271/ab271833/Images/ab271833-1-ab32527251143anticcr7antibodyy59immunofluorescence.jpg "Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y59] to CCR7 - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CCR7 antibody [Y59] - BSA and Azide free
See all CCR7 primary antibodies -
Description
Rabbit monoclonal [Y59] to CCR7 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Jurkat cells. IP: MCF7 whole cell lysate.
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General notes
ab271833 is the carrier-free version of ab32527. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y59 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)
Immunocytochemistry/Immunofluorescence analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32527). -
![Immunoprecipitation - Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)](https://www.abcam.com/ps/products/271/ab271833/Images/ab271833-2-ab32527251237anticcr7antibodyy59immunoprecipitation.jpg)
Immunoprecipitation - Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF7 (human breast adenocarcinoma cell line) whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32527). -
![Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)](https://www.abcam.com/ps/products/271/ab271833/Images/ab271833-3-benefits-of-recombinant-antibodies.png)
Anti-CCR7 antibody [Y59] - BSA and Azide free (ab271833)
