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Anti-CCR9 antibody [7E7] (ab52590)

Key features and details

  • Rat monoclonal [7E7] to CCR9
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-CCR9 antibody [7E7]
    See all CCR9 primary antibodies

  • Description

    Rat monoclonal [7E7] to CCR9

  • Host species

    Rat

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide:

    PTDFTSPIPNMADDYGSEST

    , corresponding to N terminal amino acids 3-22 of Mouse CCR9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 5% Fetal calf serum
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    7E7

  • Myeloma

    P3x63-Ag8.653

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (ab52590)
    Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (ab52590)

    ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.

    Red = CCR9

    Green = IgA

    Blue = CD3

  • Flow Cytometry - Anti-CCR9 antibody [7E7] (ab52590)
    Flow Cytometry - Anti-CCR9 antibody [7E7] (ab52590)
    Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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