Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)
![Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)](https://www.abcam.com/ps/products/269/ab269572/Images/ab269572-368340-anti-ccr7-antibody-4b12-flow-cytometry-splenocytes-mouse.jpg "Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)")
Key features and details
- Rat monoclonal [4B12] to CCR7 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
-
Product name
Anti-CCR7 antibody [4B12] - BSA and Azide free
See all CCR7 primary antibodies -
Description
Rat monoclonal [4B12] to CCR7 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
-
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: Mouse splenocytes.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269572 is the carrier-free version of ab52602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4B12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)
C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-CCR7 [4B12] antibody (ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab52602).
