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Neuroscience Cell Type Marker Neuron marker Synapse marker

Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)

Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10812] to rSec6 - BSA and Azide free
  • Suitable for: ICC, IHC-P, IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-rSec6 antibody [EPR10812] - BSA and Azide free
    See all rSec6 primary antibodies
  • Description

    Rabbit monoclonal [EPR10812] to rSec6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249263 is the carrier-free version of ab156568.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10812
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Images

  • Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Western blot - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 87 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab156568, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Immunoprecipitation - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Immunocytochemistry - Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    This data was developed using ab156568, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.
  • Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)
    Anti-rSec6 antibody [EPR10812] - BSA and Azide free (ab249263)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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