Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-rSec6 antibody [EPR10812] (ab156568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10812] to rSec6
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-rSec6 antibody [EPR10812]
    See all rSec6 primary antibodies

  • Description

    Rabbit monoclonal [EPR10812] to rSec6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human rSec6. The exact sequence is proprietary.
    Database link: O60645

  • Positive control

    • HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10812

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)
    Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)
    All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : MCF7 lysate
    Lane 3 : HepG2 lysate
    Lane 4 : fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 87 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)
    Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.
  • Anti-rSec6 antibody [EPR10812] (ab156568)
    Anti-rSec6 antibody [EPR10812] (ab156568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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