Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-rSec6 antibody [EPR10812] (ab156568)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10812] to rSec6
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-rSec6 antibody [EPR10812]
See all rSec6 primary antibodies
Description

Rabbit monoclonal [EPR10812] to rSec6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human rSec6. The exact sequence is proprietary.
Database link: O60645
Positive control
- HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10812
Isotype

IgG
Research areas

Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)

All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

Lane 1 : HeLa lysate
Lane 2 : MCF7 lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 87 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)

Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)

Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.
Anti-rSec6 antibody [EPR10812] (ab156568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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