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Syntaxin 1a ELISA Kit (ab277088)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 36 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell Lysate, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Syntaxin 1a ELISA Kit
    See all Syntaxin 1a kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 6.7%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 10.1%

  • Sample type

    Tissue Extracts, Cell Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    36 pg/ml

  • Range

    78.13 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 113 111% - 116%
    Cell Lysate 97 88% - 104%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    Syntaxin 1a ELISA kit (ab277088) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human/Mouse/Rat Syntaxin 1a protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Syntaxin 1a with 36 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Syntaxin 1a is part of the SNARE complex, which plays a critical role in neurotransmission. With its partners, SNAP25 and VAMP2, it is responsible for regulating calcium ion channels and for fusion of synaptic vesicles with the presynaptic membrane. This protein shares homology across Mammalia, and therefore is expected to cross-react with many species. The antibodies in this kit were raised against the human full-length recombinant protein.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Syntaxin 1a Capture Antibody 1 x 600µl
    10X Syntaxin 1a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    Syntaxin 1a Lyophilized Recombinant Protein 2 vials
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.

  • Tissue specificity

    Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.

  • Involvement in disease

    Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

  • Sequence similarities

    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.

  • Cellular localization

    Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt

  • Alternative names

    • HPC 1
    • Neuron specific antigen HPC1
    • Neuron-specific antigen HPC-1
    • OTTHUMP00000174615
    • OTTHUMP00000174616
    • OTTHUMP00000174617
    • OTTHUMP00000174618
    • P35-1
    • STX1
    • STX1A
    • STX1A_HUMAN
    • SYN1A
    • Syntaxin 1A (brain)
    • Syntaxin 1A brain
    • Syntaxin-1A
    see all

  • Database links

    see all

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of [Status] Syntaxin 1a standard curve in 1X Cell Extraction Buffer PTR.
    Example of [Status] Syntaxin 1a standard curve in 1X Cell Extraction Buffer PTR.

    The Syntaxin 1a standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Syntaxin 1a in 80 µg/mL SHSY-5Y cell extract, 20 µg/mL mouse brain tissue extract and 15 µg/mL rat brain tissue extract.
    Interpolated concentrations of native Syntaxin 1a in 80 µg/mL SHSY-5Y cell extract, 20 µg/mL mouse brain tissue extract and 15 µg/mL rat brain tissue extract.

    The concentrations of Syntaxin 1a were measured in duplicate and interpolated from the Syntaxin 1a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Syntaxin 1a concentration was determined to be 3,353.80 pg/mL in SHSY-5Y cell extract, 2,463.89 pg/mL in mouse brain tissue extract and 3,289.96 pg/mL in rat brain tissue extract.

  • Sandwich ELISA - Syntaxin 1a ELISA Kit
    Sandwich ELISA - Syntaxin 1a ELISA Kit

    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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