Syntaxin 1a Antibody Pair - BSA and Azide free (ab253475)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Syntaxin 1a Antibody Pair - BSA and Azide free
See all Syntaxin 1a kits -
Assay type
ELISA set -
Range
78.125 pg/ml - 5000 pg/ml -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Syntaxin 1a. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277088), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Syntaxin 1a Capture Antibody (unconjugated) 1 x 100µg Syntaxin 1a Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
Tissue specificity
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. -
Involvement in disease
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Alternative names
- HPC 1
- Neuron specific antigen HPC1
- Neuron-specific antigen HPC-1
see all -
Database links
- Entrez Gene: 6804 Human
- Entrez Gene: 20907 Mouse
- Entrez Gene: 116470 Rat
- Omim: 186590 Human
- SwissProt: Q16623 Human
- SwissProt: O35526 Mouse
- SwissProt: P32851 Rat
- Unigene: 647024 Human
see all
Images
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Sandwich ELISA - Syntaxin 1a Antibody Pair - BSA and Azide free (ab253475)To learn more about the advantages of recombinant antibodies see here.
