Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
![Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)](https://www.abcam.com/ps/products/170/ab170889/Images/ab170889-170704-anti-syntaxin-1a-antibody-epr11073b-immunoprecipitation.jpg "Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Syntaxin 1a antibody [EPR11073(B)]
See all Syntaxin 1a primary antibodies -
Description
Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Syntaxin 1a aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q16623 -
Positive control
- Human cerebellum and fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11073(B) -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
Western blot analysis on immunoprecipitation pellet from human fetal brain lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170889 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170889, 1/1000 dilution
Sample: Immunoprecipitated Human fetal brain lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170889, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgGPredicted band 33 kDa
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![Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)](https://www.abcam.com/ps/products/170/ab170889/Images/ab170889-170702-anti-syntaxin-1a-antibody-epr11073b-western-blot.jpg)
Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)All lanes : Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
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![Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)](https://www.abcam.com/ps/products/170/ab170889/Images/ab170889-3-benefits-of-recombinant-antibodies.png)
Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
