Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Syntaxin 1a antibody [EPR11073(B)]
    See all Syntaxin 1a primary antibodies

  • Description

    Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Syntaxin 1a aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16623

  • Positive control

    • Human cerebellum and fetal brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
    Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)

    Western blot analysis on immunoprecipitation pellet from human fetal brain lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170889 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

     

    Lane 1 Primary: ab170889, 1/1000 dilution
    Sample: Immunoprecipitated Human fetal brain lysate
    Secondary: HRP-conjugated anti-rabbit IgG

     

    Lane 2 Primary: ab170889, 1/1000 dilution
    Sample: 1X PBS (negative control)
    Secondary: HRP-conjugated anti-rabbit IgG

     

     

    Predicted band 33 kDa

  • Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
    Western blot - Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
    All lanes : Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa

  • Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)
    Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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