Anti-Syntaxin 1a antibody (ab41453)
Key features and details
- Rabbit polyclonal to Syntaxin 1a
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 1a antibody
See all Syntaxin 1a primary antibodies -
Description
Rabbit polyclonal to Syntaxin 1a -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Macaque monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Rat Syntaxin 1a aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab41452) -
Positive control
- ICC/IF: primary hippocampal rat neurons/glia, DIV14. cells, and PC12 cells. WB: Mouse Brain, Rat Brain, Human Brain tissue lysates. IP: Mouse Brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Images
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Western blot - Anti-Syntaxin 1a antibody (ab41453)All lanes : Anti-Syntaxin 1a antibody (ab41453) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Brain (Rat) Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody (ab41453)ICC/IF image of ab41453 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41453, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunoprecipitation - Anti-Syntaxin 1a antibody (ab41453)Syntaxin 1a was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Syntaxin 1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41453.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 33kDa, non specific band - 25kDa: We are unsure as to the identity of this extra band; Syntaxin 1a
