Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDLIM7 antibody [EPR13171] (ab182153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13171] to PDLIM7
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PDLIM7 antibody [EPR13171]
    See all PDLIM7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13171] to PDLIM7

  • Host species

    Rabbit

  • Specificity

    The immunogen sequence for ab182153 shares 86% homology with the mouse PDLIM7 protein however in our hands this antibody did not work in mouse and rat samples in western blot (no bands detected). In some human cell lines, in addition to the band of interest detected at 50 kDa, this product also bound to a protein at ~37-40 kDa in weight. For best results it may be preferable to use a harsh lysis protocol for preparation of cell or tissue lysates. Please see notes in the western blot image below.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and Jurkat whole cell lysate (ab7899); Human infiltrating ductal breast carcinoma tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDLIM7 antibody [EPR13171] (ab182153)
    Western blot - Anti-PDLIM7 antibody [EPR13171] (ab182153)
    Lane 1 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/10000 dilution
    Lanes 2-4 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/2000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 : WI-38 (human lung) whole cell lysate
    Lane 4 : SK-OV-3 (human adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST

    Dilution buffer:5% NFDM/TBST

    Lane 1 exposure time: 1 minute

    Lanes 2 - 4 exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] (ab182153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] (ab182153)

    Immunohistochemical analysis of paraffin-embedded Human infiltrating ductal breast carcinoma tissue labeling PDLIM7 with ab182153 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PDLIM7 antibody [EPR13171] (ab182153)
    Flow Cytometry - Anti-PDLIM7 antibody [EPR13171] (ab182153)

    Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PDLIM7 with ab182153 at 1/60 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-PDLIM7 antibody [EPR13171] (ab182153)
    Anti-PDLIM7 antibody [EPR13171] (ab182153)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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