Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22259] to CCR6 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CCR6 antibody [EPR22259] - BSA and Azide free
    See all CCR6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22259] to CCR6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HL-60, Hut-78 and HUVEC whole cell lysates; Human tonsil and thymus tissue lysate. IHC-P: Human Hodgkin's lymphoma and tonsil tissue. IP: HL-60 whole cell lysate.

  • General notes

    Ab243852 is the carrier-free version of ab227036. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243852 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human tonsil (PMID: 12514792) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human Hodgkin’s lymphoma (PMID: 12514792) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

  • Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
    Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)

    CCR6 was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with ab227036 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab227036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Lane 1: HL-60 whole cell lysate 10 µg (input).
    Lane 2: ab227036 IP in HL-60 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227036 in HL-60 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

  • Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
    Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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