Anti-CCR8 antibody (ab63772)
Key features and details
- Rabbit polyclonal to CCR8
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CCR8 antibody
See all CCR8 primary antibodies -
Description
Rabbit polyclonal to CCR8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide corresponding to Mouse CCR8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab66424) -
Positive control
- This antibody gave a positive signal in the following lysates: SK N SH Whole Cell, A498 Whole Cell, HCT 116 Whole Cell, Mouse Spinal Cord Tissue, Mouse Thymus Tissue, Rat Thymus Tissue, Mouse Spleen Tissue, Rat Spleen Tissue and MEF1 Whole Cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Target
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Function
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1237 Human
- Entrez Gene: 12776 Mouse
- Omim: 601834 Human
- SwissProt: P51685 Human
- SwissProt: P56484 Mouse
- Unigene: 113222 Human
- Unigene: 442098 Mouse
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Alternative names
- C C chemokine receptor type 8 antibody
- C C CKR 8 antibody
- C C motif chemokine receptor 8 antibody
see all
Images
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All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml
Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Thymus (Rat) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : Spleen (Rat) Tissue Lysate
Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
References (0)
ab63772 has not yet been referenced specifically in any publications.
Images
-
All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml
Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
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All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Thymus (Rat) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : Spleen (Rat) Tissue Lysate
Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds