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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)

Anti-CCR8 antibody (ab63772)

Anti-CCR8 antibody (ab63772)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CCR8
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CCR8 antibody
    See all CCR8 primary antibodies
  • Description

    Rabbit polyclonal to CCR8
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse CCR8 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab66424)

  • Positive control

    • This antibody gave a positive signal in the following lysates: SK N SH Whole Cell, A498 Whole Cell, HCT 116 Whole Cell, Mouse Spinal Cord Tissue, Mouse Thymus Tissue, Rat Thymus Tissue, Mouse Spleen Tissue, Rat Spleen Tissue and MEF1 Whole Cell

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CCR8 protein (ab158145)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab63772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P51685 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1237 Human
    • Entrez Gene: 12776 Mouse
    • Omim: 601834 Human
    • SwissProt: P51685 Human
    • SwissProt: P56484 Mouse
    • Unigene: 113222 Human
    • Unigene: 442098 Mouse
    • Alternative names

      • C C chemokine receptor type 8 antibody
      • C C CKR 8 antibody
      • C C motif chemokine receptor 8 antibody
      • C-C chemokine receptor type 8 antibody
      • C-C CKR-8 antibody
      • CC chemokine receptor 8 antibody
      • CC chemokine receptor CHEMR1 antibody
      • CC chemokine receptor type 8 antibody
      • CC CKR 8 antibody
      • CC-CKR-8 antibody
      • CCR 8 antibody
      • CCR-8 antibody
      • Ccr8 antibody
      • CCR8 protein antibody
      • CCR8-L antibody
      • CCR8_HUMAN antibody
      • CDw198 antibody
      • CDw198 antigen antibody
      • Chemokine (C C motif) receptor 8 antibody
      • Chemokine (C C) receptor 8 antibody
      • Chemokine (C C) receptor like 2 antibody
      • Chemokine (CC motif) receptor 8 antibody
      • Chemokine (CC) receptor 8 antibody
      • Chemokine (CC) receptor like 1 antibody
      • Chemokine (CC) receptor like 2 antibody
      • Chemokine C C motif receptor 8 antibody
      • Chemokine C C receptor 8 antibody
      • Chemokine CC motif receptor 8 antibody
      • Chemokine CC receptor 8 antibody
      • Chemokine receptor 8 antibody
      • Chemokine receptor like 1 antibody
      • Chemokine receptor-like 1 antibody
      • CKR L1 antibody
      • CKR-L1 antibody
      • CKRL 1 antibody
      • CKRL1 antibody
      • CMKBR 8 antibody
      • CMKBR L2 antibody
      • CMKBR8 antibody
      • CMKBRL 2 antibody
      • CMKBRL2 antibody
      • CY 6 antibody
      • CY6 antibody
      • GPR CY6 antibody
      • GPR-CY6 antibody
      • GPRCY6 antibody
      • MGC123958 antibody
      • MGC123959 antibody
      • MGC129966 antibody
      • MGC129973 antibody
      • TER 1 antibody
      • TER1 antibody
      see all

    Images

    • Western blot - Anti-CCR8 antibody (ab63772)
      Western blot - Anti-CCR8 antibody (ab63772)
      All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml

      Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
      Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
      Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
      Lane 4 : Spinal Cord (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-CCR8 antibody (ab63772)
      Western blot - Anti-CCR8 antibody (ab63772)
      All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml

      Lane 1 : Thymus (Mouse) Tissue Lysate
      Lane 2 : Thymus (Rat) Tissue Lysate
      Lane 3 : Spleen (Mouse) Tissue Lysate
      Lane 4 : Spleen (Rat) Tissue Lysate
      Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab63772? Please let us know so that we can cite the reference in this datasheet.

    ab63772 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-CCR8 antibody (ab63772)
      Western blot - Anti-CCR8 antibody (ab63772)
      All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml

      Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate
      Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
      Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
      Lane 4 : Spinal Cord (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-CCR8 antibody (ab63772)
      Western blot - Anti-CCR8 antibody (ab63772)
      All lanes : Anti-CCR8 antibody (ab63772) at 1 µg/ml

      Lane 1 : Thymus (Mouse) Tissue Lysate
      Lane 2 : Thymus (Rat) Tissue Lysate
      Lane 3 : Spleen (Mouse) Tissue Lysate
      Lane 4 : Spleen (Rat) Tissue Lysate
      Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa
      Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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