Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E77] to CCR8
Suitable for: ELISA, WB, IP
Reacts with: Human, Recombinant fragment

Product name

Anti-CCR8 antibody [E77]
See all CCR8 primary antibodies
Description

Rabbit monoclonal [E77] to CCR8
Host species

Rabbit
Specificity

Does not cross-react with other G-protein coupled receptor 1 family members.

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human CCR8.
Epitope

Residues in loop 2 extracellular domain of human CCR8
Positive control
- WB: Human Thymus and spleen tissue lysates; A431 cell lysate IP: Human thymus tissue lysate
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E77
Isotype

IgG
Research areas

Western blot - Anti-CCR8 antibody [E77] (ab32399)

Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa

Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-CCR8 antibody [E77] (ab32399)

ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST .
ELISA - Anti-CCR8 antibody [E77] (ab32399)

ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
Western blot - Anti-CCR8 antibody [E77] (ab32399)

Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa
Western blot - Anti-CCR8 antibody [E77] (ab32399)

Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa
Anti-CCR8 antibody [E77] (ab32399)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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