Anti-CCR8 antibody [E77] (ab32399)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E77] to CCR8
- Suitable for: ELISA, WB, IP
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-CCR8 antibody [E77]
See all CCR8 primary antibodies -
Description
Rabbit monoclonal [E77] to CCR8 -
Host species
Rabbit -
Specificity
Does not cross-react with other G-protein coupled receptor 1 family members. -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentIP HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human CCR8.
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Epitope
Residues in loop 2 extracellular domain of human CCR8 -
Positive control
- WB: Human Thymus and spleen tissue lysates; A431 cell lysate IP: Human thymus tissue lysate
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E77 -
Isotype
IgG -
Research areas
Images
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Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
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Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
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Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
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