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Human CXCL1 ELISA Kit (GRO alpha) (ab100530)

Key features and details

  • Sensitivity: 2 pg/ml
  • Range: 2 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CXCL1 ELISA Kit (GRO alpha)
    See all CXCL1/GRO alpha kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2 pg/ml

  • Range

    2 pg/ml - 1000 pg/ml

  • Recovery

    > 99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112.2 101% - 122%
    Serum 130.4 119% - 145%
    Plasma 113.5 85% - 140%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA® range.


     

  • Notes

    Optimization may be required with urine samples

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human GRO-alpha 2 vials
    GRO-alpha Microplate (12 x 8 wells) 1 unit
    Recombinant Human GRO-alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • CXCL1
    • Cytokine-induced neutrophil chemoattractant 1
    • Fibroblast secretory protein
    • Fsp
    • Gro
    • Gro 1
    • Gro A
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO-alpha(6-73)
    • Gro1
    • Gro1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • GROA_HUMAN
    • Growth-regulated alpha protein
    • KC
    • KC chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • Melanoma growth stimulatory activity, alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • N51
    • NAP-3
    • NAP3
    • Neutrophil-activating protein 3
    • Platelet-derived growth factor-inducible protein KC
    • Scyb 1
    • Scyb1
    • Secretory protein N51
    • Small inducible cytokine subfamily B, member 1
    see all

  • Database links

    Images

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100530

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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