Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.064 pg/ml
- Range: 2.34 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CXCL1 ELISA Kit (GRO alpha)
See all CXCL1/GRO alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 3 1.9% Inter-assay Sample n Mean SD CV% Supernatant 8 3.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.064 pg/ml -
Range
2.34 pg/ml - 150 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 92% - 97% Serum 83 82% - 85% Hep Plasma 86 85% - 87% Cit plasma 81 80% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CXCL1 ELISA Kit (ab190805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL1 with 0.064 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CXCL1 Capture Antibody 1 x 600µl 10X Human CXCL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CXCL1 Lyophilized Recombinant Protein (ab9806) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
Database links
- Entrez Gene: 2919 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- Unigene: 708652 Human
- Unigene: 789 Human
Images
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Other - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CXCL1 (GRO alpha) Standard Curve Comparison.Standard Curve comparison between human CXCL1 SimpleStep ELISA kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.Standard Curve comparison between new human CXCL1 SimpleStep ELISA kit (GRO alpha) and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
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Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL1 (GRO alpha) in human serum, plasma and samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, platelet poor plasma (citrate) 100%, and platelet poor plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 20.65 pg/mL in serum, 11.10 pg/mL in platelet poor plasma (citrate), and 29.39 pg/mL in platelet poor plasma (Heparin).
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Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 17.53 ng/mL in treated PBMC supernatant.
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Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells were cultured for 2 days at 37˚C in the presence or absence of 1.5% PHA-M. The concentrations of CXCL1 (GRO alpha) were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean CXCL1 concentration was determined to be 5.11 pg/mL in unstimulated PBMC supernatants, and 17533.21 pg/mL in stimulated PBMC supernatants.
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Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)To learn more about the advantages of recombinant antibodies see here.
