Anti-ATF6 antibody - Carboxyterminal end (ab62576)
Key features and details
- Rabbit polyclonal to ATF6 - Carboxyterminal end
- Suitable for: ICC/IF, ICC, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ATF6 antibody - Carboxyterminal end
See all ATF6 primary antibodies -
Description
Rabbit polyclonal to ATF6 - Carboxyterminal end -
Host species
Rabbit -
Specificity
ab62576 only reacts with only ATF6 alpha. -
Tested applications
Suitable for: ICC/IF, ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 16 amino acid peptide from near the carboxy terminus of human ATF6
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Positive control
- ICC/IF: MCF7 cells. WB: EL4 cell lysate. IHC-P: Human breast tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.
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Paraffin-embedded human breast tissue stained for ATF6 using ab62576 at 5 µg/ml in immunohistochemical analysis.
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ab62576 at 10µg/ml staining AFT6 in MCF7 cells by Immunocytochemistry.
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Lane 1 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 0.5 µg/ml
Lane 2 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 1 µg/ml
All lanes : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
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Immunofluorescence of ATF6 in MCF7 cells using ab62576 at 10 ug/ml.