Anti-ATF6 antibody (ab174756)
Key features and details
- Goat polyclonal to ATF6
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF6 antibody
See all ATF6 primary antibodies -
Description
Goat polyclonal to ATF6 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ATF6 aa 437-449 (internal sequence) (Cysteine residue).
Sequence:NSYRYDHSVSNDK
Database link: P18850 -
Positive control
- WB: HeLa and MCF7 whole cell lysate; HeLa and Jurkat nuclear cell lysate; ICC: A431 and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATF6 antibody (ab174756)All lanes : Anti-ATF6 antibody (ab174756) at 1 µg/ml (primary incubation: 1 hour at room temperature)
Lane 1 : HeLa whole cell lysate in RIPA buffer
Lane 2 : MCF7 whole cell lysate in RIPA buffer
Lane 3 : HeLa nuclear cell lysate in RIPA buffer
Lane 4 : Jurkat nuclear cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 75-80 kDa why is the actual band size different from the predicted?
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Immunocytochemistry - Anti-ATF6 antibody (ab174756)Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells labelling ATF6 with ab174756 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunocytochemistry - Anti-ATF6 antibody (ab174756)Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labelling ATF6 with ab174756 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
