Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BATF antibody [EPR21911-55] (ab236876)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21911-55] to BATF
  • Suitable for: ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BATF antibody [EPR21911-55]
    See all BATF primary antibodies

  • Description

    Rabbit monoclonal [EPR21911-55] to BATF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: KARPAS-299 and A20 whole cell lysate. ICC/IF: KARPAS-299 cells. Flow Cyt: KARPAS-299 cells. IP: KARPAS-299 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BATF antibody [EPR21911-55] (ab236876)
    Western blot - Anti-BATF antibody [EPR21911-55] (ab236876)
    All lanes : Anti-BATF antibody [EPR21911-55] (ab236876) at 1/1000 dilution

    Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa


    Exposure time: 3 minutes


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa (PMID:8570175).

  • Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (ab236876)
    Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (ab236876)

    BATF was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab236876 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236876 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 µg (Input).
    Lane 2: ab236876 IP in KARPAS-299 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236876 in KARPAS-299 whole cell lysate (-).


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    We used a high sensitivity ECL substrate to develop this blot.

  • Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (ab236876)
    Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (ab236876)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF with ab236876 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining in KARPAS-299 cell line is observed.

    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The control is KARPAS-299 cell line stained incubated with secondary antibody only (ab150077 AlexaFluor®488 Goat anti-Rabbit secondary).

    Negative control: HeLa cell line（PMID: 8570175).

  • Flow Cytometry - Anti-BATF antibody [EPR21911-55] (ab236876)
    Flow Cytometry - Anti-BATF antibody [EPR21911-55] (ab236876)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line (left) and KARPAS-299 (human anaplastic large cell lymphoma) cell line (right) labeling BATF with ab236876 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa （PMID: 8570175).

  • Anti-BATF antibody [EPR21911-55] (ab236876)
    Anti-BATF antibody [EPR21911-55] (ab236876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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