Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BATF antibody [EPR21911-55] (ab236876)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21911-55] to BATF
Suitable for: ICC/IF, IP, Flow Cyt, WB
Reacts with: Mouse, Human

Product name

Anti-BATF antibody [EPR21911-55]
See all BATF primary antibodies
Description

Rabbit monoclonal [EPR21911-55] to BATF
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: KARPAS-299 and A20 whole cell lysate. ICC/IF: KARPAS-299 cells. Flow Cyt: KARPAS-299 cells. IP: KARPAS-299 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21911-55
Isotype

IgG
Research areas

Western blot - Anti-BATF antibody [EPR21911-55] (ab236876)

All lanes : Anti-BATF antibody [EPR21911-55] (ab236876) at 1/1000 dilution

Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 14 kDa

Exposure time: 3 minutes

Blocking/diluting buffer and concentration: 5% NFDM/TBST.

Negative control: HeLa (PMID:8570175).
Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (ab236876)

BATF was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab236876 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236876 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 µg (Input).
Lane 2: ab236876 IP in KARPAS-299 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236876 in KARPAS-299 whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.

We used a high sensitivity ECL substrate to develop this blot.
Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (ab236876)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF with ab236876 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining in KARPAS-299 cell line is observed.

Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The control is KARPAS-299 cell line stained incubated with secondary antibody only (ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary).

Negative control: HeLa cell line（PMID: 8570175).
Flow Cytometry - Anti-BATF antibody [EPR21911-55] (ab236876)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line (left) and KARPAS-299 (human anaplastic large cell lymphoma) cell line (right) labeling BATF with ab236876 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control: HeLa （PMID: 8570175).
Anti-BATF antibody [EPR21911-55] (ab236876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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