Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E268] to ATF2 (phospho T71) - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free
    See all ATF2 primary antibodies

  • Description

    Rabbit monoclonal [E268] to ATF2 (phospho T71) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, Dot blotmore details
    Unsuitable for: Flow Cyt or IHC

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate. ICC/IF: HeLa cells treated with 250ng/ml anisomycin for 30min.

  • General notes

    ab242381 is the carrier-free version of ab32019 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab242381 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
    Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)

    Lane 1 (input): HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate  10μg.
    Lane 2 (+): HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.
    Lane 3 (-):  Rabbit monoclonal IgG (ab172730) instead of ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.

    ab32019 Immunoprecipitating ATF2 in Human Hela whole cell lysate. For western blotting ab32019 (1:1000) was used to confirm successful immunoprecipitation. Blocking and diluting buffer used was 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32019).

  • Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
    Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)

    Immunocytochemistry of HeLa (Human epithelial cell line from cervix adenocarcinoma), prepared in FBS free medium overnight labeling ATF2 at 0.9 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Alexa Fluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/500. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counter stain at 2.5 μg/ml. DAPI was used for nuclear counter stain. Confocal image showing the expression was increased on HeLa cells, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30min.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32019).

  • Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
    Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)

    Dot blot analysis of human ATF2 (phospho T71) peptide (Lane 1) and human ATF2 non-phospho peptide (Lane 2) using ab32019 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Exposure time: 3 minutes

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32019).

  • Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
    Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)

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