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Anti-HIF-1 alpha antibody [54/HIF-1a] (ab279654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [54/HIF-1a] to HIF-1 alpha
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HIF-1 alpha antibody [54/HIF-1a]
    See all HIF-1 alpha primary antibodies

  • Description

    Mouse monoclonal [54/HIF-1a] to HIF-1 alpha

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with 1 mM DFO (Deferoxamine mesylate) for 24 hrs, whole cell lysate. HeLa treated with 0.5 mM CoCl2 for 6 hrs, whole cell lysate. ICC: HeLa cells treated with 1 mM desferrioxamine for 24 hrs.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-HIF-1 alpha antibody [54] (ab279654)
    Western blot - Anti-HIF-1 alpha antibody [54] (ab279654)
    All lanes : Anti-HIF-1 alpha antibody [54/HIF-1a] (ab279654) at 1/2000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa treated with 1 mM DFO (Deferoxamine mesylate) for 24 hours whole cell lysate
    Lane 3 : Untreated HeLa whole cell lysate
    Lane 4 : HeLa treated with 0.5 mM CoCl2 for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/50000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 9159130).

    Exposure time: 3 minutes.

  • Immunocytochemistry - Anti-HIF-1 alpha antibody [54] (ab279654)
    Immunocytochemistry - Anti-HIF-1 alpha antibody [54] (ab279654)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HIF-1 alpha with ab279654 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing nuclear staining in HeLa cells treated with 1 mM desferrioxamine for 24 hrs.

    ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only controls: Secondary antibodies are ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution and ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

  • Anti-HIF-1 alpha antibody [54/HIF-1a] (ab279654)
    Anti-HIF-1 alpha antibody [54/HIF-1a] (ab279654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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