PP 1, Src family tyrosine kinase inhibitor (ab120859)
Key features and details
- Potent Src family tyrosine kinase inhibitor
- CAS Number: 172889-26-8
- Purity: > 98%
- Soluble in DMSO to 25 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PP 1, Src family tyrosine kinase inhibitor -
Description
Potent Src family tyrosine kinase inhibitor -
Purity
> 98% -
CAS Number
172889-26-8 -
Chemical structure
Properties
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Chemical name
1-(1,1-Dimethylethyl)-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine -
Molecular weight
281.36 -
Molecular formula
C16H19N5 -
PubChem identifier
1400 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM and in ethanol to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C -
Source
Synthetic
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Research areas
Images
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Functional Studies - PP 1, Src family tyrosine kinase inhibitor (ab120859) McGee et al PLoS One. 2017 Nov 30;12(11):e0188645. doi: 10.1371/journal.pone.0188645. eCollection 2017. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Herpes simplex virus-2 regulates ER-alpha and HA via Src kinase in ECT-1 cells.
(Panel B) ECT-1 cells were treated with vehicle (DMSO) or Src inhibitor (SKI/PP1). ER-alpha was quantified using densitometry following Western blot analysis (ANOVA with Tukey’s multiple comparison; NT vs VEH, n.s.; NT vs SKI/PPI, p
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Functional Studies - PP 1, Src family tyrosine kinase inhibitor (ab120859)ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
