Anti-HIF1 beta antibody (ab232964)
Key features and details
- Rabbit polyclonal to HIF1 beta
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HIF1 beta antibody
See all HIF1 beta primary antibodies -
Description
Rabbit polyclonal to HIF1 beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Cow
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human HIF1 beta aa 600 to the C-terminus. (Expressed in E.coli).
Database link: P27540 -
Positive control
- WB: Recombinant human HIF1 beta protein; Human serum; HepG2 cell lysate; Pig kidney lysate; Rat placenta lysate. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232964 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIF1 beta antibody (ab232964)All lanes : Anti-HIF1 beta antibody (ab232964) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Pig kidney lysate
Lane 4 : Rat placenta lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 87 kDa
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Western blot - Anti-HIF1 beta antibody (ab232964)Anti-HIF1 beta antibody (ab232964) at 2 µg/ml + Recombinant human HIF1 beta protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 87 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody (ab232964)HepG2 (human liver hepatocellular carcinoma cell line) cells labelling HIF1 beta (green) using ab232964 at 20 µg/ml in ICC/IF, followed by FITC staining.
