Recombinant Human HIF-1 alpha protein (ab154478)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: ELISA, WB, SDS-PAGE
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Product name
Recombinant Human HIF-1 alpha protein
See all HIF-1 alpha proteins and peptides -
Purity
> 75 % SDS-PAGE.
ab154478 was purified by Ni chromatography and sterile filtered. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEY QGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQL KEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPD LGTENLYFQGMEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYEL AHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNC FYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCD HEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWK VLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSK TFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKT HHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNY VVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKL KKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPN EKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFT MPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLF AEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASP ESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIA SPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNV LSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIG -
Predicted molecular weight
100 kDa including tags -
Amino acids
1 to 735 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.61% Tris, 10% Glycerol, 0.88% Sodium chloride
Images
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SDS-PAGE - Recombinant Human HIF-1 alpha protein (ab154478)
1µg of ab154478 was examined by SDS-PAGE and commassie blue protein stain. Full-length HIF-1-alpha is indicated by the arrow. Purity is judged to be >75%.
Expected MW is 100kDa.
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Western blot - Recombinant Human HIF-1 alpha protein (ab154478)ab154478 was examined by western blot with an anti-HIF-1-alpha antibody.
Lane1: 10ng HIF1 alpha full-length protein (ab154478)
Block: 4% milk/PBS Primary antibody: anti-HIF-1-alpha (ab51608), 1:1000; 2 hours room temperature. Secondary antibody: anti-Rabbit HRP 1:5000 ECL detection
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ELISA - Recombinant Human HIF-1 alpha protein (ab154478)ab154478 was tested in the HIF1A Human ELISA Kit (ab117996). ab154478 was tested under standard conditions in the sandwich ELISA kit using a 3-fold dilution series from 3µg/ml.
