Human CCL14 ELISA Kit (HCC-1) (ab272201)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.4 pg/ml
- Range: 5.5 pg/ml - 350 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CCL14 ELISA Kit (HCC-1)
See all CCL14 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.6% Inter-assay Sample n Mean SD CV% Serum 4 5.1% -
Sample type
Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.4 pg/ml -
Range
5.5 pg/ml - 350 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 88 83% - 94% Urine 101 99% - 102% Serum 111 108% - 118% Cell culture media 98 95% - 100% Hep Plasma 102 101% - 104% EDTA Plasma 112 109% - 117% Cit plasma 112 108% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CCL14 ELISA kit (ab272201) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL14 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL14 with 4.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
C-C motif chemokine 14 (CCL14), also known as HCC-1 and NCC-2, is a cytokine encoded by the gene CCL14. Mature secreted CCL14 is a 74- amino acid containing active glycoprotein and is constitutively expressed by multiple tissues and has been found at high concentrations in plasma.
CCl14 enhances the proliferation of CD34 myeloid progenitor cells and has weak activities on human monocytes through receptors that also recognize MIP-1 alpha. CCL14 induces intracellular Ca2+ changes and enzyme release but does not induce chemotaxis in monocytes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CCL14 (HCC-1) Capture Antibody 1 x 600µl 10X Human CCL14 (HCC-1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CCL14 (HCC-1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. -
Tissue specificity
Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsThe N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CC-1
- CC-3
- CCL14
see all -
Database links
- Entrez Gene: 6358 Human
- Omim: 601392 Human
- SwissProt: Q16627 Human
- Unigene: 272493 Human
- Unigene: 740501 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CCL14 standard curve in Sample Diluent NS.The CCL14 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CCL14 in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (EDTA) 1:200, plasma (citrate) 1:200, plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 59.0 ng/mL in serum, 43.1 ng/mL in plasma (EDTA), 48.8 ng/mL in and plasma (citrate), and 98.9 ng/mL in plasma (heparin).
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Interpolated concentrations of native CCL14 in human saliva and urine.The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum, plasma (EDTA), plasma – (citrate), plasma – (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 96.7 pg/mL in saliva and 87.9 pg/ml in urine.
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Serum from eight individual healthy CCL14 donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL14 concentration was determined to be 33.3 ng/mL with a range of 20.5 – 56.5 ng/mL.
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Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)To learn more about the advantages of recombinant antibodies see here.
