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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-Utrophin antibody (ab95443)

Anti-Utrophin antibody (ab95443)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Utrophin
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Utrophin antibody
    See all Utrophin primary antibodies
  • Description

    Rabbit polyclonal to Utrophin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Utrophin aa 800-900 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107901)

  • Positive control

    • This antibody gave a positive signal in Human Skeletal muscle tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Utrophin peptide (ab107901)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Utrophin protein (ab159789)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab95443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Dog
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 394 kDa (predicted molecular weight: 394 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 394 kDa (predicted molecular weight: 394 kDa).

Target

  • Function

    May play a role in anchoring the cytoskeleton to the plasma membrane.
  • Tissue specificity

    Muscle.
  • Sequence similarities

    Contains 2 CH (calponin-homology) domains.
    Contains 20 spectrin repeats.
    Contains 1 WW domain.
    Contains 1 ZZ-type zinc finger.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Neuromuscular junction.
  • Target information above from: UniProt accession P46939 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 431687 Dog
    • Entrez Gene: 442965 Dog
    • Entrez Gene: 7402 Human
    • Omim: 128240 Human
    • SwissProt: P46939 Human
    • SwissProt: Q5SZ57 Human
    • SwissProt: Q5T097 Human
    • Unigene: 133135 Human
    • Unigene: 7402 Human
    see all
  • Alternative names

    • DMDL antibody
    • DRP 1 antibody
    • DRP antibody
    • DRP-1 antibody
    • DRP1 antibody
    • Dystrophin like protein antibody
    • Dystrophin related protein 1 antibody
    • Dystrophin related protein antibody
    • Dystrophin-related protein 1 antibody
    • FLJ23678 antibody
    • OTTHUMP00000040139 antibody
    • UTRN antibody
    • UTRO_HUMAN antibody
    • Utrophin (homologous to dystrophin) antibody
    • Utrophin antibody
    see all

Images

  • Western blot - Anti-Utrophin antibody (ab95443)
    Western blot - Anti-Utrophin antibody (ab95443)
    Anti-Utrophin antibody (ab95443) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 394 kDa
    Observed band size: 394 kDa
    Additional bands at: 215 kDa, 31 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab95443? Please let us know so that we can cite the reference in this datasheet.

    ab95443 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-Utrophin antibody (ab95443)
      Western blot - Anti-Utrophin antibody (ab95443)
      Anti-Utrophin antibody (ab95443) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 394 kDa
      Observed band size: 394 kDa
      Additional bands at: 215 kDa, 31 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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