Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)
![Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)](https://www.abcam.com/ps/products/250/ab250776/Images/ab250776-369111-anti-hif1-beta-antibody-epr17516-bsa-and-azide-free-western-blot-hap1-wt-hap1-arnt-knockout-hela-hepg2.jpg "Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17516] to HIF1 beta - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free
See all HIF1 beta primary antibodies -
Description
Rabbit monoclonal [EPR17516] to HIF1 beta - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 and HeLa whole cell lysates.
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General notes
ab250776 is the carrier-free version of ab184711. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250776 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17516 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)All lanes : Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ARNT knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184711).
Lanes 1 - 4: Merged signal (red and green). Green - ab184711 observed at 90 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab184711 was shown to react with ARNT in HAP1 wild-type cells in Western blot. Loss of signal was observed when ARNT knockout sample was used. HAP1 wild-type and ARNT knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab184711 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)](https://www.abcam.com/ps/products/250/ab250776/Images/ab250776-2-benefits-of-recombinant-antibodies.png)
Anti-HIF1 beta antibody [EPR17516] - BSA and Azide free (ab250776)
