HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)
![HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)](https://www.abcam.com/ps/products/205/ab205833/Images/ab205833-265788-anti-hif-1-alpha-antibody-ep1215y-hrp-western-blot.jpg "HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1215Y] to HIF-1 alpha
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-HIF-1 alpha antibody [EP1215Y]
See all HIF-1 alpha primary antibodies -
Description
HRP Rabbit monoclonal [EP1215Y] to HIF-1 alpha -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB : HeLa DFO treated nuclear lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 2.24 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1215Y -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)All lanes : HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205833 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)](https://www.abcam.com/ps/products/205/ab205833/Images/ab205833-3-benefits-of-recombinant-antibodies.png)
HRP Anti-HIF-1 alpha antibody [EP1215Y] (ab205833)
