Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16897] to HIF-1 alpha - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free
    See all HIF-1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR16897] to HIF-1 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Ferret, Non human primates

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate treated with 0.5mM CoCl2 for 6 hours; NIH/3T3 cell lyaste treated with 0.1mM CoCl2 for 24 hours. ICC/IF: Hela cells treated with DFO (1 mM, 24 h).

  • General notes

    ab221610 is the carrier-free version of ab179483 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab221610 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)
    Western blot - Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)
    All lanes : Anti-HIF-1 alpha antibody [EPR16897] (ab179483) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Wild type HAP1 treated with DMOG (0.5mM 18hr) whole cell lysate
    Lane 3 : HIF1A knockout HAP1 whole cell lysate
    Lane 4 : HIF1A knockout HAP1 treated with DMOG (0.5mM 18hr) whole cell lysate
    Lane 5 : HeLa whole cell lysate
    Lane 6 : HeLa treated with DMOG (0.5mM 18hr) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 92 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab179483 observed at 105 kDa. Red - loading control, ab24834, observed at 50 kDa.

    ab179483 was shown to specifically react with HIF-1 alpha in wild-type HAP1 treated DMOG (0.5mM 18hr) cells as signal was lost in HAP1 knockout treated DMOG (0.5mM 18hr) knockout cells. Wild-type and HAP1 knockout samples were subjected to SDS-PAGE. ab179483 and ab24834 (Mouse anti-Histone H3 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179483).

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIF-1-alpha with ab179483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cells treated with DFO (1 mM, 24 h). The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (anti-alpha Tubulin mouse mAb) (Alexa Fluor® 594) at 1/200 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179483).

  • Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)
    Anti-HIF-1 alpha antibody [EPR16897] - BSA and Azide free (ab221610)

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For licensing inquiries, please contact partnerships@abcam.com

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