Anti-HIF-1 alpha antibody (ab216842)
Key features and details
- Rabbit polyclonal to HIF-1 alpha
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HIF-1 alpha antibody
See all HIF-1 alpha primary antibodies -
Description
Rabbit polyclonal to HIF-1 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF MouseIHC-P HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human HIF-1 alpha aa 350-450 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q16665 -
Positive control
- IHC-P: Human cervical carcinoma tissue. WB: Mouse heart lysates; rat brain and spleen tissue lysates; HL60 cell lysate. ICC/IF: KLN 205 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIF-1 alpha antibody (ab216842)All lanes : Anti-HIF-1 alpha antibody (ab216842) at 1/200 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse large intestine lysate
Secondary
All lanes : Goat anti-rabbit IgG antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 92 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody (ab216842)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling HIF-1-alpha with ab216842 at 1/300 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody (ab216842)Immunofluorescent analysis of KLN 205 (mouse) cells labeling HIF-1-alpha with ab216842 at 1/50 dilution followed by an FITC conjugated secondary antibody.
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Western blot - Anti-HIF-1 alpha antibody (ab216842)All lanes : Anti-HIF-1 alpha antibody (ab216842) at 1/1000 dilution
Lanes 1-2 : Rat spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat brain tissue lysate
Predicted band size: 92 kDa
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Western blot - Anti-HIF-1 alpha antibody (ab216842)Anti-HIF-1 alpha antibody (ab216842) at 1/300 dilution + HL60 cell lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 92 kDa
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Flow Cytometry - Anti-HIF-1 alpha antibody (ab216842)Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling HIF-1 aplha with ab216842 at a 1/100 dilution for 30 minutes (green) compared to control cells (blue) and an isotype control (orange).
