Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-124-3F7] to HIF-1 alpha
Suitable for: WB, IHC-P, ICC, ChIP-sequencing, IP
Reacts with: Human

Product name

Anti-HIF-1 alpha antibody [BL-124-3F7]
See all HIF-1 alpha primary antibodies
Description

Rabbit monoclonal [BL-124-3F7] to HIF-1 alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP-seq	Human
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human HIF-1 alpha aa 433-826. NP_001521.1 and Gene ID 3091.
Database link: Q16665
Positive control
- ChIPseq: CoCl2 treated Hep-G2 cell lysate. WB: CoCl2 treated Hep-G2 and HeLa cell lysates. IHC-P: Human prostate carcinoma tissue. IP: CoCl2 treated Hep-G2 and HeLa cell lysates. ICC: CoCl2 treated HepG2 cells.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-124-3F7
Isotype

IgG
Research areas

ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Localization of human HIF1-alpha binding sites in immunoprecipitates from CoCl2 treated Hep-G2 lysate by ChIP-Seq using ab243860
Western blot - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

All lanes : Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Lane 1 : 200M CoCl2 treated Hep-G2 cell lysate
Lane 2 : Mock treated Hep-G2 cell lysate
Lane 3 : 200M CoCl2 treated HeLa cell lysate
Lane 4 : Mock treated HeLa cell lysate

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HIF-1 alpha using ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

HIF-1 alpha was immunoprecipitated from 1mg CoCl2 and mock treated HepG2 and HeLa whole cell lysates with ab243860 at 20µg/mL lysate.

All lanes : Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860) at 1/1000 dilution

Lane 1 : IP using ab243860 in 200µM CoCl2 treated HepG2 cell lysate
Lane 2 : IP using ab243860 in mock treated HepG2 cell lysate
Lane 3 : IP using ab243860 in 200µM CoCl2 treated HeLa cell lysate
Lane 4 : IP using ab243860 in mock treated HeLa cell lysate
Lane 5 : IP using Control IgG in 200µM CoCl2 treated HepG2 cell lysate

Exposure time: 30 seconds
Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-1 alpha using ab243860 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-1 alpha using ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-HIF-1 alpha antibody [BL-124-3F7] (ab243860)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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