Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)
![Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)](https://www.abcam.com/ps/products/241/ab241997/Images/ab241997-331811-97b.jpg "Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)")
Key features and details
- Mouse monoclonal [S393-2] to Spectrin beta chain, brain 3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Spectrin beta chain, brain 3 antibody [S393-2]
See all Spectrin beta chain, brain 3 primary antibodies -
Description
Mouse monoclonal [S393-2] to Spectrin beta chain, brain 3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human Spectrin beta chain, brain 3 aa 1600-1850. (C-terminal repeats 14 to 15)
Database link: Q9H254 -
Positive control
- WB: COS cells over-expressing human Spectrin beta chain, brain 3-His protein. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S393-2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997) at 1/1000 dilution + COS cells over-expressing human Spectrin beta chain, brain 3-His protein at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 289 kDaPrimary incubation for 16 hrs at 4°C.
Secondary incubation for 1 hr at RT.
Blocked for 1 hr at RT.
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![Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)](https://www.abcam.com/ps/products/241/ab241997/Images/ab241997-331810-97a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Spectrin beta chain, brain 3 antibody [S393-2] (ab241997)4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Spectrin beta chain, brain 3 (green, C) using ab241997 at 1/100 dilution for 60 minutes at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
