Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF-1 alpha antibody [54/HIF-1a] - BSA and Azide free (ab279658)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [54/HIF-1a] to HIF-1 alpha - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HIF-1 alpha antibody [54/HIF-1a] - BSA and Azide free
    See all HIF-1 alpha primary antibodies

  • Description

    Mouse monoclonal [54/HIF-1a] to HIF-1 alpha - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with 1 mM DFO (Deferoxamine mesylate) for 24 hrs, whole cell lysate. HeLa treated with 0.5 mM CoCl2 for 6 hrs, whole cell lysate. ICC: HeLa cells treated with 1 mM desferrioxamine for 24 hrs.

  • General notes

    ab279658 is the carrier-free version of ab279654. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279658 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-HIF-1 alpha antibody [54] - BSA and Azide free (ab279658)
    Western blot - Anti-HIF-1 alpha antibody [54] - BSA and Azide free (ab279658)
    All lanes : Anti-HIF-1 alpha antibody [54/HIF-1a] (ab279654) at 1/2000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa treated with 1 mM DFO (Deferoxamine mesylate) for 24 hrs, whole cell lysate
    Lane 3 : Untreated HeLa whole cell lysate
    Lane 4 : HeLa treated with 0.5 mM CoCl2 for 6 hrs, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/50000 dilution

    Predicted band size: 92 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab279654, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 9159130).

    Exposure time: 3 mins.

  • Immunocytochemistry - Anti-HIF-1 alpha antibody [54] - BSA and Azide free (ab279658)
    Immunocytochemistry - Anti-HIF-1 alpha antibody [54] - BSA and Azide free (ab279658)

    This data was developed using ab279654 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HIF-1 alpha with ab279654 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing nuclear staining in HeLa cells treated with 1 mM desferrioxamine for 24 hrs.

    ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only controls: Secondary antibodies are ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution and ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

  • Anti-HIF-1 alpha antibody [54/HIF-1a] - BSA and Azide free (ab279658)
    Anti-HIF-1 alpha antibody [54/HIF-1a] - BSA and Azide free (ab279658)

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