Anti-HIF-1 alpha antibody (ab82832)
Key features and details
- Rabbit polyclonal to HIF-1 alpha
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HIF-1 alpha antibody
See all HIF-1 alpha primary antibodies -
Description
Rabbit polyclonal to HIF-1 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human HIF-1 alpha conjugated to keyhole limpet haemocyanin.
Database link: Q16665 -
General notes
Under normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia treated samples as positive control (ab180880). Hypoxia can be induced with treatment using certain agents e.g. CoCl2 or DFO, etc. so proper sample preparation is critical.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Crude serum, final bleed. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIF-1 alpha antibody (ab82832)All lanes : Anti-HIF-1 alpha antibody (ab82832) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
Secondary
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 93 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Molecular weight of tag is 14kDa.
