Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10058(3)] to TNFAIP8 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free
    See all TNFAIP8 primary antibodies

  • Description

    Rabbit monoclonal [EPR10058(3)] to TNFAIP8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251212 is the carrier-free version of ab195810. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251212 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    All lanes : Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 19,21 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab195810, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

  • Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution + Human fetal spleen lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 19,21 kDa why is the actual band size different from the predicted?



    This data was developed using ab195810, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

  • Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    This data was developed using ab195810, the same antibody clone in a different buffer formulation.TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract (Input) 10µg. Lane 2: ab195810 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)
    Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (ab251212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.