Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23106-55] to HIF1 beta
  • Suitable for: WB, IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HIF1 beta antibody [EPR23106-55]
    See all HIF1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR23106-55] to HIF1 beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    ICC/IF
    Mouse
    Human
    IP
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 and HeLa lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Western blot - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    All lanes : Anti-HIF1 beta antibody [EPR23106-55] (ab239366) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 87 kDa
    Observed band size: 87 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1:26 seconds; Lane 2:37 seconds.

    Lysates should be made fresh and used in WB immediately to minimize protein degradation.

  • Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    HIF1 beta was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab239366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239366 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab239366 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239366 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

     

  • Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Immunoprecipitation - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    HIF1 beta was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab239366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239366 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab239366 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239366 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

     

  • Flow Cytometry - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Flow Cytometry - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling HIF1 beta with ab239366 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Flow Cytometry - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling HIF1 beta with ab239366 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling HIF1 beta with ab239366 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling HIF1 beta with ab239366 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000  dilution (Green). Confocal image showing mainly nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Anti-HIF1 beta antibody [EPR23106-55] (ab239366)
    Anti-HIF1 beta antibody [EPR23106-55] (ab239366)

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