Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha
Suitable for: ICC/IF, IP, ChIP-sequencing, ICC, IHC-P, WB
Reacts with: Human

Product name

Anti-HIF-2-alpha antibody [BL-95-1A2]
See all HIF-2-alpha primary antibodies
Description

Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP-seq	Human
ICC	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HIF-2-alpha aa 400-450. The exact sequence is proprietary. NP_001421.2 and Gene ID 2034.
Database link: Q99814
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-95-1A2
Isotype

IgG
Research areas

ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Localization of human HIF2-alpha binding sites in immunoprecipitates from subcutaneous tumor 786-O cells by ChIP-Seq using ab243861.
Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.
Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution.
Lane 1: ab243861 IP in HepG2 cell lysate treated with 200 µM CoCl2.

Lane 2: ab243861 IP in HepG2 whole cell lysate Mock treated.
Lane 3: Contol IgG in HepG2 cell lysate treated with 200 µM CoCl2.
Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Detection of human HIF2-alpha by WB of Hep-G2, HEK293T, and 786-O lysate treated with 200 M CoCl2 (+) or mock treated (-) using ab243861. Secondary: HRP-conjugated goat anti-rabbit IgG.
Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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