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Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22370-51] to D Box Binding Protein - BSA and Azide free
  • Suitable for: IP, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free
    See all D Box Binding Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR22370-51] to D Box Binding Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human hypothalamus and brain lysates; HEK-293T transfected with D Box Binding Protein with a C-terminal myc-His tag.ICC/IF: 293T cells. Flow Cyt: HEK-293T (transfected with myc-his tagged D Box Binding Protein expression vector) cells. IP: HEK-293T (transfected with myc-His tagged D Box Binding Protein expression vector) cell lysate.

  • General notes

    Ab263022 is the carrier-free version of ab222112. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab263022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
    Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)

    D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.

    Lane 2: ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

  • Flow Cytometry - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
    Flow Cytometry - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Secondary antibody is Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution.

    Cells were stained with rabbit IgG (Left) or ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

  • Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
    Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with ab222112 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222112).

  • Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)
    Anti-D Box Binding Protein antibody [EPR22370-51] - BSA and Azide free (ab263022)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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