Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22370-51] to D Box Binding Protein
  • Suitable for: WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-D Box Binding Protein antibody [EPR22370-51]
    See all D Box Binding Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR22370-51] to D Box Binding Protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human hypothalamus and brain lysates; HEK-293T transfected with D Box Binding Protein with a C-terminal myc-His tag. ICC/IF: 293T cells. Flow Cyt: HEK-293T (transfected with myc-his tagged D Box Binding Protein expression vector) cells. IP: HEK-293T (transfected with myc-His tagged D Box Binding Protein expression vector) cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22370-51

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    All lanes : Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human hypothalamus lysate
    Lane 6 : Human brain lysate
    Lane 7 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The DBP protein is ubiquitously expressed but mainly at suprachiasmatic nuclei in the hypothalamus or liver, and fine-tuned by circadian clock. The antibody detects a band around the reported molecular weight of DBP in human hypothalamus and brain tissue lysates. Negative control: testis (PMID: 2331750)

    Exposure time: 3 minutes.

  • Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Western blot - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    All lanes : Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with D Box Binding Protein (WT) expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Immunoprecipitation - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)

    D Box Binding Protein was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector, whole cell lysate, with ab222112 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab222112 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate 10ug.

    Lane 2: ab222112 IP in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222112 in HEK-293T transfected with myc-His tagged D Box Binding Protein expression vector whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

     

  • Flow Cytometry - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Flow Cytometry - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-his tagged D Box Binding Protein expression vector) cells labelling D Box Binding Protein with ab222112 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Secondary antibody is Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution.

    Cells were stained with rabbit IgG (Left) or ab222112 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.

  • Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Immunocytochemistry/ Immunofluorescence - Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling D Box Binding Protein with ab222112 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with myc-tagged D Box Binding Protein expression vector. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)
    Anti-D Box Binding Protein antibody [EPR22370-51] (ab222112)

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