Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-7 antibody [EPR17029] (ab181579)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17029] to Caspase-7
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Caspase-7 antibody [EPR17029]
    See all Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR17029] to Caspase-7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse heart, kidney and spleen tissue lysates. C6, RAW 264.7, PC-12 and NIH/3T3 cell lysates. Rat brain, heart, kidney and spleen tissue lysates. ICC/IF and IP: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : C6 (Rat glial tumor cells) lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35,32,27 kDa
    why is the actual band size different from the predicted?



    Observed band size: 35kDa band is the pro-Caspase 7, 32kDa band is the N-terminal propeptide cleaved Caspase 7, and the 27kDa band is an N-terminal truncated caspase 7.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa



    Observed band size: The 35kDa band is the pro-caspase 7; 32kDa band is the N-terminal propeptide cleaved caspase 7; the 27kDa band is the truncated form and the 11kDa band is Caspase 7 subunit p11.
    Lanes 1-4 were from the same blot; the exposure time for lanes 1-3 is 1 min and for lane 4 is 2 min.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [EPR17029] (ab181579)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Caspase-7 with ab181579 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab181579 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

    Please see Pubmed IDs: 22464733 and 11829465 for further information on Caspase-7 localization.

  • Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] (ab181579)
    Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] (ab181579)

    Caspase-7 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) with ab181579 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab181579 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: NIH/3T3 whole cell extract. Lane 2: PBS instead of extract.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed band at 35kDa band is the pro-caspase-7.

  • Anti-Caspase-7 antibody [EPR17029] (ab181579)
    Anti-Caspase-7 antibody [EPR17029] (ab181579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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