Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Caspase-7 antibody [EPR17029] (ab181579)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17029] to Caspase-7
Suitable for: ICC/IF, IP, WB
Reacts with: Mouse, Rat

Product name

Anti-Caspase-7 antibody [EPR17029]
See all Caspase-7 primary antibodies
Description

Rabbit monoclonal [EPR17029] to Caspase-7
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse heart, kidney and spleen tissue lysates. C6, RAW 264.7, PC-12 and NIH/3T3 cell lysates. Rat brain, heart, kidney and spleen tissue lysates. ICC/IF and IP: NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17029
Isotype

IgG
Research areas

Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)

All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : C6 (Rat glial tumor cells) lysate
Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 35,32,27 kDa
why is the actual band size different from the predicted?

Observed band size: 35kDa band is the pro-Caspase 7, 32kDa band is the N-terminal propeptide cleaved Caspase 7, and the 27kDa band is an N-terminal truncated caspase 7.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Caspase-7 antibody [EPR17029] (ab181579)

All lanes : Anti-Caspase-7 antibody [EPR17029] (ab181579) at 1/5000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: The 35kDa band is the pro-caspase 7; 32kDa band is the N-terminal propeptide cleaved caspase 7; the 27kDa band is the truncated form and the 11kDa band is Caspase 7 subunit p11.
Lanes 1-4 were from the same blot; the exposure time for lanes 1-3 is 1 min and for lane 4 is 2 min.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-7 antibody [EPR17029] (ab181579)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling Caspase-7 with ab181579 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on NIH/3T3 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab181579 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Please see Pubmed IDs: 22464733 and 11829465 for further information on Caspase-7 localization.
Immunoprecipitation - Anti-Caspase-7 antibody [EPR17029] (ab181579)

Caspase-7 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) with ab181579 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab181579 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: NIH/3T3 whole cell extract. Lane 2: PBS instead of extract.
Blocking/Dilution buffer: 5% NFDM/TBST.
The observed band at 35kDa band is the pro-caspase-7.
Anti-Caspase-7 antibody [EPR17029] (ab181579)

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