Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Caspase-7 antibody [4D10B2] (ab201959)

Key features and details

Mouse monoclonal [4D10B2] to Caspase-7
Suitable for: IHC-P, WB, Flow Cyt
Knockout validated
Reacts with: Rat, Human
Isotype: IgG1

Product name

Anti-Caspase-7 antibody [4D10B2]
See all Caspase-7 primary antibodies
Description

Mouse monoclonal [4D10B2] to Caspase-7
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Caspase-7 aa 1-200. (Purified from E.coli).
Database link: P55210

Run BLAST with Run BLAST with
Positive control
- Human Caspase-7 (aa29-198) recombinant protein; Caspase-7 (aa29-198)-hIgGFc transfected HEK293 cell lysate; Jurkat, HEK293, MOLT4, MCF 7 and PC-12 cell lysates; MCF7 cells; Human cervical cancer tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

4D10B2
Isotype

IgG1
Research areas

Western blot - Anti-Caspase-7 antibody [4D10B2] (ab201959)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HAP1 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
Lane 3: CASP7 whole cell lysate (20 µg)
Lane 4: CASP7 + Staurosporin whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)

Lanes 1 - 6: Merged signal (red and green). Green - ab201959 observed at 38,20 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab201959 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab201959 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Caspase-7 antibody [4D10B2] (ab201959)

Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution + Human Caspase-7 (aa29-198) recombinant protein

Predicted band size: 34 kDa
Observed band size: 22 kDa
why is the actual band size different from the predicted?

Expected MWt is 22.5 kDa.
Western blot - Anti-Caspase-7 antibody [4D10B2] (ab201959)

All lanes : Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : Caspase-7 (aa29-198)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 34 kDa
Western blot - Anti-Caspase-7 antibody [4D10B2] (ab201959)

All lanes : Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : PC12 cell lysate

Predicted band size: 34 kDa
Flow Cytometry - Anti-Caspase-7 antibody [4D10B2] (ab201959)

Flow cytometric analysis of MCF7 cells labeling Caspase -7 with ab201959 at 1/200 dilution (green) compared to a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody [4D10B2] (ab201959)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Caspase-7 with ab201959 at 1/200 dilution.

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