Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22717-11] to Caspase-11 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free
    See all Caspase-11 primary antibodies

  • Description

    Rabbit monoclonal [EPR22717-11] to Caspase-11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type iBMDM treated with /ml LPS.

  • General notes

    ab270036 is the carrier-free version of ab246496. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab270036 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22717-11

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)
    Western blot - Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)
    All lanes : Anti-Caspase-11 antibody [EPR22717-11] (ab246496) at 1/1000 dilution

    Lane 1 : Wild-type iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate
    Lane 2 : Caspase-11 knockout iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab246496, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This product is specific to full length Caspase-11.

    The image was kindly provided by the collaborator.

    ab246496 Anti-Caspase-11 antibody [EPR22717-11] was shown to react with Caspase-11 in iBMDM cells in Western blot. Loss of signal was observed when Caspase-11 knockout sample was used. Wild-type and Caspase-11 knockout samples were subjected to SDS-PAGE. ab246496 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.

    Exposure time: 1 minute

  • Western blot - Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)
    Western blot - Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)
    All lanes : Anti-Caspase-11 antibody [EPR22717-11] (ab246496) at 1/1000 dilution

    Lane 1 : Wild-type iBMDM whole cell lysate
    Lane 2 : Wild-type iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Observed band size: 43 kDa why is the actual band size different from the predicted?



    This data was developed using ab246496, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This product is specific to full length.

    The lysates were kindly provided by the collaborator.

    Exposure time: 3 minutes

  • Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)
    Anti-Caspase-11 antibody [EPR22717-11] - BSA and Azide free (ab270036)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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